Genomic Technologies Facility Plant Sciences Institute Iowa State University

Genomic Technologies Facility › Services

2025 Roy J. Carver Co-Laboratory, Iowa State University
1111 WOI Road, Ames, Iowa 50011-1085
Email:

Dear GTF customers,

Due to COVID-19 concerns, CCL no longer have front desk students. If you need to access GTF during business hours, please send your name and ISU ID number to GTF Staff (gtfstaff@iastate.edu). We will request CCL building access for you and you should be able to enter the building using your ISU ID few days after we request access from FPM.

Sorry for any inconvenience this may cause you and thank you for your patience during this time!

GTF Staff

High Throughput Nucleic Acid Manipulation

Plant DNA Extraction:

GTF provides high throughput DNA Extraction using the Qiagen BioSprint96. Qiagen DNA Extraction is suitable for Ion Torrent or Illumina Sequencing and Sequence Capture. Once the lysate is prepared, a single plate can be extracted in 30 minutes. Extraction can be conducted by on-campus customers who undergo training to become certified to run the instrument themselves. Grinding and quantification services are not included in the price of a run, but GTF can provide this service for an additional fee. Certified users must provide their own pipets and tips. The BioSprint is available to customers for use 8 AM to 5 PM Monday through Friday when the University is in session. Please contact GTF staff if services are required outside of these times. User Forms are available to be printed and filled out prior to your scheduled appointment in the GTF or a printed copy is available by the instrument to be filled out at the time of your appointment.

Plant RNA Extraction:

GTF provides high-throughput RNA Extraction using the Qiagen BioSprint96. With the BioSprint, 96 samples can be extracted in 30 minutes. BioSprint extraction can be conducted by on-campus customers who undergo training to become certified to run the instrument themselves. Grinding and quantification services are not included in the price for a run, but GTF can provide this service for an additional fee. Certified users must provide their own pipets and tips. The BioSprint is available to customers for use 8 AM to 5 PM Monday through Friday when the University is in session. Please if services are required outside of these times. User Forms are available to be printed and filled out prior to your scheduled appointment in the GTF or a printed copy is available by the instrument to be filled out at the time of your appointment.

Eppendorf AF2200 Plate Reader:

GTF offers the AF2200 Plate Reader for nucleic acid quantification in a high-throughput format. The Eppendorf µPlate 0.5 is available for measuring the absorbance of small volume samples. GTF supplies both absorbance and fluorescence filters for use. Users must provide their own supplies and consumables (pipets, tips, tubes, plates, reagents, etc.). The Plate Reader is available to customers for use 8 AM to 5 PM Monday through Friday when the University is in session. Please contact GTF staff if services are required outside of these times.

Miscellaneous Services and Instruments

Sage Science's BluePippin:

GTF provides the Blue Pippin for customers for use in the collection of DNA fragments. 1.5% Agarose gel cassettes contain 5 wells with run times of 30-100 minutes. 1.5% gel cassettes are able to target 250bp - 1.5kb fragments and 2% gel cassettes target 100-600 bp fragments. Other cassette sizes may be available for purchase; please contact GTF staff before planning your experiment. A one-hour training session with GTF staff is required for new users. The Blue Pippin is available to customers for use 8 AM to 5 PM Monday through Friday when the University is in session. Please contact GTF staff if services are required outside of these times. Users must provide their own consumables (pipet tips, tubes, etc.) User Forms are available to be printed and filled out prior to your scheduled appointment in the GTF or a printed copy is available by the instrument to be filled out at the time of your appointment.

Agilent Bioanalyzer:

GTF provides an Agilent Bioanalyzer for customers to use with the DNA 1000, DNA 7500, DNA High Sensitivity, RNA 6000 Nano, and RNA 6000 Pico Chips. A one-hour training session with GTF staff is required for new users. The Bioanalyzer is available to customers for use 8 AM to 5 PM Monday through Friday when the University is in session. Please contact GTF staff if services are required outside of these times. Users must provide their own consumables (pipet tips, tubes, etc.). User Forms are available to be printed and filled out prior to your scheduled appointment in the GTF or a printed copy is available by the instrument to be filled out at the time of your appointment.

Eppendorf epMotion M5073:

GTF offers the Eppendorf epMotion M5073 automated liquid handling system for routine pipetting uses. The epMotion system is equipped with 50 µL and 1000 µL high precision dispensing pipettes, a ThermoMixer and ThermoRack, and a MagSep module that can be utilized for separation and nucleic acid purifications. A one-hour training session with GTF staff is required for new users. Users may purchase certified tips from the facility, and use the instrument at no additional cost. Any additional supplies needed must be provided by the user. The epMotion is available for customers for use 8 AM to 5 PM Monday through Friday when the University is in session. Please contact GTF staff if services are required outside of these times.