Center for Plant Genomics
Center for Plant Genomics Plant Sciences Institute Iowa State University

SAM Chips Comparison

Below is the table summarizing the informative, unique, and total spots of each of the SAM chips produced at our facility. Comparison was conducted by evaluating the unique GeneBank Accession numbers of the ESTs in each microarray chip.

Chip Name GPL # Total Spots Informative
Spots
Unique Spots
SAM 1.0 GPL2557 19,200 14,401 14,374
SAM 1.1 GPL3333 19,200 14,401 14,374
SAM 1.2 GPL4521 15,680 14,118 14,117
SAM 2.0 GPL2572 14,080 8,991 8,986
SAM 3.0 GPL3538 16,000 14,270 13,892

Further computational comparisons were performed using the unique spots among all the chips to determine the number of unique ESTs that are present in each chip as described in the tables and Venn diagrams below. For details of the EST accession numbers and spot locations, please refer to the excel spreadsheet found here.

INTERSECTION OF UNIQUE SPOTS AMONG ALL SAM CHIPS
Chip Name SAM 1.0
(GPL2557)
SAM 1.1
(GPL3333)
SAM 1.2
(GPL4521)
SAM 2.0
(GPL2572)
SAM 3.0
(GPL3538)
SAM 1.0
(GPL2557)
14,374  
SAM 1.1
(GPL3333)
14,374 14,374  
SAM 1.2
(GPL4521)
11,363 11,363 14,117  
SAM 2.0
(GPL2572)
118 118 891 8,986  
SAM 3.0
(GPL3538)
1,891 1,891 984 873 13,892
Intersection of Unique Spots in SAM 1.0, 1.1, & 1.2 Intersection of Spots in SAM 1.2, 20, & 3.0