Genomic Technologies Facility - Services
2025 Roy J. Carver Co-Laboratory
515-294-7491
The GTF has a Cartesian PixSys 5500 Arrayer with an automated microtiter plate stacker/destacker to generate microarrays, a Lucidea SlidePro Hybridizer for microarray slide hybridization and a Perkin Elmer ProScan Array HT Microarray Analysis System capable of detecting four distinct fluorescent signals from a given microarray. Users provide their own gene targets for the chips and prepare their own chips. It is also possible to spot other materials (e.g., proteins or carbohydrates). The Facility also provides slide spotting and scanning on a fee-for-service basis. Researchers who wish to use maize cDNA arrays, developed by the Schnable Laboratory, may contact the facility manager.
If you wish to process Affymetrix GeneChips, please visit http://www.biotech.iastate.edu/facilities/genechip/Genechip.htm
The MassARRAY® system offers a suite of applications for quantitative and qualitative genomic analysis via MALDI-TOF mass spectrometry. iPLEX® is a SNP genotyping technology that can multiplex up to 40 SNPs in one reaction and provides up to 150,000 genotypes per day. Users provide the sequences of their targets of interest for assay design and GTF staff will confirm the design with users. Assays will be conducted by GTF staff.
A set (1,016) SNP markers developed by the Schnable Lab that distinguish the maize inbred lines B73 and Mo17 are available at GTF for use in a wide variety of maize genetic experiments. These validated SNP markers were used to generate the 291RI SNP Map. These SNPs can be used for Bulk Segregation Analysis (BSA, Liu et al., Genetics, in press) The 1,016 SNPs are now efficiently divided into 30 groups for genetic mapping experiments such as BSA. Six to twelve mutants can be mapped on each Sequenom chip. Bioinformatics analysis of BSA data is available from the GTF.
Genomic Technologies Facility User/Billing Agreements
For on-campus users 
For off-campus users
Facility Fee Structure
Interim Sequenom User Agreement
For more information, please contact GTF staff at 515-294-7491, or by writing to .